Senior/Advanced Molecular Technologist Position Overview:
We are seeking an experienced and highly motivated Senior/Advanced Molecular Technologist that is passionate about Next Generation Sequencing (NGS) to join a progressive and innovative genomics laboratory team. The successful candidate will play a pivotal role in the implementation, execution, optimization, and QC of high-throughput genomic sequencing assays. This individual will possess at least 4 years of hands-on experience in Whole genome sequencing (WGS) technologies, including indepth knowledge ofIllumina sequencing technology and ideally, additional experience in Oxford Nanopore Technologies (ONT).
This position requires a strong background in molecular biology, bioinformatics, and NGS workflows, with a focus on ensuring accuracy, efficiency, and compliance with laboratory standards and regulatory requirements. You will be responsible for conducting complex genomic assays, data interpretation, quality control, and contributing to the ongoing development of sequencing methodologies and pipeline improvements.
Key Responsibilities:
NGS Assay Development and Execution:
- Perform and oversee whole genome sequencing (WGS) using Illumina platforms (e.g., NovaSeq, NextSeq, etc.).
- Optimize and implement protocols for Oxford Nanopore Technologies (ONT), including preparation, sequencing, and data acquisition.
- Troubleshoot technical issues related to library preparation, sequencing runs, and sample quality.
- Ensure proper sample tracking, handling, and processing in accordance with laboratory standard operating procedures (SOPs).
Data Analysis & Interpretation:
- Collaborate with bioinformaticians and data scientists to analyze WGS data, ensuring high-quality variant detection, annotation, and reporting.
- Conduct initial quality control (QC) checks of sequencing data and identify trends or anomalies that may impact results.
- Interpret sequencing data for various applications, including germline and somatic variant detection, structural variant analysis, and genome assembly.
Technology & Process Improvement:
- Identify and drive improvements in existing NGS workflows to enhance throughput, accuracy, and cost-efficiency.
- Stay up-to-date with emerging trends and technologies in NGS, including improvements in long-read sequencing and pangenomic analysis.
- Work with R&D and bioinformatics teams to evaluate and implement new genomic assays and technologies.
Regulatory Compliance & Quality Control:
- Maintain compliance with CLIA, CAP, and other relevant regulatory standards.
- Ensure accurate and timely documentation of all experimental data, protocol deviations, and quality control measures.
- Participate in internal and external audits and proficiency testing as required.
Training & Mentorship:
- Provide training and technical guidance to junior technologists, laboratory staff, and interns.
- Lead and mentor a team of molecular technologists to ensure adherence to best practices and the highest level of technical expertise.
- Develop educational resources and training modules for staff to stay current with evolving NGS techniques.
Qualifications:
Education:
- Bachelor’s degree, Master’s degree, or PhD in Molecular Biology, Genomics, Bioinformatics, or a related field.
- Advanced certifications in molecular diagnostics or genomics (ASCP, MB(ASCP), etc.) are preferred.
- California Clinical Laboratory Scientist (CLS) License or eligibility for certification.
Experience:
- Minimum 4 years of hands-on experience with Next Generation Sequencing (NGS), particularly in whole genome sequencing.
- Demonstrated proficiency with Illumina sequencing platforms (e.g., NovaSeq, NextSeq, MiSeq).
- Experience with Oxford Nanopore Technologies (ONT) is highly desirable.
- Proven expertise in WGS quality control processes.
- Previous experience working in CLIA- and/or CAP-certified environments is a plus.
Skills:
- Strong understanding of molecular biology, NGS workflows, and bioinformatics principles.
- Familiarity with genomic data analysis tools such as bcl2fastq, FASTQC, GATK, SAMtools, and other variant calling pipelines.
- Ability to work independently and collaboratively in a fast-paced environment with cross-functional teams.
- Excellent problem-solving, communication, and organizational skills.
- Demonstrated ability to lead projects, manage timelines, and meet stringent quality standards.
Preferred Experience:
- Working knowledge of long-read sequencing technologies (ONT, PacBio).
- Experience in pangenomic references and the use of advanced genomic tools for structural variation and haplotype phasing.
- Familiarity with methylation analysis and polygenic risk scores (PRS).
Benefits:
- Competitive salary, benefits package, and equity commensurate with position and salary.
- Significant opportunities for professional growth and involvement in pioneering genomic research.
- Work in a state-of-the-art laboratory using cutting-edge sequencing technologies.
